Mass Estimation of Native Proteins by Blue Native Electrophoresis
نویسندگان
چکیده
منابع مشابه
Mass estimation of native proteins by blue native electrophoresis: principles and practical hints.
Blue native electrophoresis is one of the most popular techniques for mass estimation of native membrane proteins, but the use of non-optimal mass markers and acrylamide gels can compromise accuracy and reliability of the results. We present short protocols taking 10-30 min to prepare optimal sets of membrane protein markers from chicken, rat, mouse, and bovine heart. Especially heart materials...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2010
ISSN: 1535-9476
DOI: 10.1074/mcp.m900526-mcp200